Nucleic Acid Engineering (2017 spring)
Research area
- Nucleic acid bioengineering
- Microarray technology
- Electrochemical diagnostics
- Nanobiotechnology
Current project
Highlights
 
HOME > Research > Research area > Microarray technology

 

DNA chip

 

Of several microarray-based methods, zip-code microarray technology offers many promising opportunities due to its unique advantages over the conventional technology. The unique and distinct short oligonucleotides that are designed for the purpose of addressing complementary target sequences on arrays are known as zip-code sequences. Most attractive feature of this approach is that once developed, the zip-code microarray can be used for different sets of human genetic mutations. Recently, by combining zip-code microarray and new assay platforms using several kinds of enzymes, we have developed various strategies for the detection of genetic mutations of MODY and BRCA. For instance, zip-code microarray systems based on single base extension (SBE), ligation chain reaction (LCR) and single strand specific nuclease assay (SSS) have been developed and their diagnostic utilities were successfully verified by correctly diagnosing various human genetic mutations in MODY and BRCA genes.

 

 

 

 

Genitourinary infectious diseases are known to occur frequently among human beings and some of them have high possibility to pass into chronic diseases. However, current treatments are carried out without exact identification of infected pathogens and antibiotic resistances, causing overdose of multiple antibiotics. For the efficient diagnosis and treatment, we developed the DNA chip to reliably identify 14 pathogens simultaneously, causing the sexually transmitted diseases. The DNA chip developed is expected to have superior performance in terms of efficiency, specificity, sensitivity, and cost-effectiveness.